葡萄糖(HK)檢測試劑盒産品說明書_平博电竞

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平博电竞

廣州偉伯科技有限公司
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葡萄糖(HK)檢測試劑盒産品說明書
2020.03.11   點擊126次

(本中文說明書為翻譯稿,僅供參考,應以英文版為準)

貨号:3091315 (Sigma-Aldrich : GAHK20)

儲存溫度:2-8℃

産品描述:

作為分析工具的酶已在食品,生化和制藥行業中得到廣泛使用。酶法具有特異性高,可重現性好,靈敏度高,反應快速的特點,因此是分析的理想選擇。由于酶的高度特異性和敏感性,因此可以在幾乎沒有樣品制備或沒有樣品制備的情況下,對粗物質進行定量測定食品和其他材料中的葡萄糖。

葡萄糖(HK)檢測試劑盒用于食品和其他材料中葡萄糖的定量、酶法測定。該試劑盒已應用于不同的系統以進行與下列課題有關的研究:

·HEK細胞中的肝葡萄糖和脂質平衡4

·産婦肥胖模型5

·纖維素酶燃料電池轉化6

原理:

 ΔA = ATest –ATotal Blank

TV = 總檢測體積 (mL)

SV = 樣品體積 (mL)

葡萄糖分子量 = 180.2 g/mole 或等效于 180.2 g/moles

F = 制樣稀釋倍數

ε= NADH 在340nm處的毫摩爾消光系【毫摩爾-1 cm-1 或等效于 (mL/μmoles)(1/cm)】

d = 光徑 (cm) = 1 cm

1,000 = 微克到毫克的換算系數

參考文獻:

1、Bondar, R.J.L., and Mead, D.C., Clin、Chem., 20(5), 586-590 (1974).

2、Kunsst, A、et al., Methods of Enzymatic Analysis, 3rd Edition (H.U、Bergmeyer, ed.)、Academic Press (New York, NY), Vol、2, pp、163-172 (1984).

3、Southgate, D.A.T., Determination of Food Carbohydrates、Applied Science Publishers (London, UK: 1976).

4、Cheng, Y.-S、et al., PLoS Genet., 11(10), e1005561 (2015).

5、Chen, J.-R、et al., Endocrinology, 157(11), 4172-4183 (2016).

6、Chen, Q、et al., J、Biotech., 263, 30-35 (2017).

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吉祥电竞

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